电玩城赠送金币50万

电玩城赠送金币50万-石贵阳-正高--

国家级重点学科

一级学科博士学位授予点

“双一流”建设学科

ENGLISH

师资队伍
Faculty and staff

吴丹

吴丹

职称:副教授、硕士生生导师

学历/学位:研究生、博士

电话:0510-85918156

电子邮箱:wudan@jiangnan.edu.cn

简介

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在电玩城赠送金币50万和食品科学与技术国家重点实验室长期从事酶工程、基因工程和发酵工程方面的研究,擅长各种微生物分子改造、蛋白质(酶)分子改造相关技术,在工业微生物发酵过程优化方面具有丰富的研究经验。2015-2016作为国家公派访问学者在美国Vanderbilt University医学院从事基因编辑研究。主持和参与国家自然科学基金、江苏省自然科学基金、国家高技术研究发展计划(863计划)等多项国家和省部级课题10余项,近年来在国内外主流学术期刊上发表学术论文40余篇,其中SCI文章共30篇,包括J Biotechnol、Appl Microb Biotech、J Agr Food Chem、Microb Cell Fact等国际权威杂志。研究成果已获授权专利10多项,其中国际专利2项。参与完成科技成果鉴定4项,2012年获教育部科技进步一等奖一项,2018年获商业联合会特等奖一项。

学习工作经历

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1994-1998,  北京工商大学,生物化工专业,工学学士;

2001-2004,  浙江大学,遗传学专业,理学硕士;

2005-2009,  华东理工大学,生物化工专业,工学博士;

2009-2018,  电玩城赠送金币50万食品科学与技术国家重点实验室,副教授;

2015-2016,  美国Vanderbilt University,访问学者;

2018至今,    电玩城赠送金币50万电玩城赠送金币50万,副教授;


主要成果

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主要代表性成果

一、           代表性论文

1.      Dan Wu, Haifeng Zhu, Ju Chu, Jing Wu*. N-acetyltransferase coexpression increases the α-glucosidase expression level in Pichia pastoris. Journal of Biotechnology, 2019, 289: 26-30.

2.      Xin Gao, Jing Wu, Dan Wu*. Rational modification of N140 and W806 amino acids of Aspergillus oryzae-derived β-galactosidase to improve its capability in galactooligosaccharide transglycoside production. Food Chemistry, 2019, 286: 362-367

3.      Miaomiao Wang, Jing Wu, Dan Wu*, Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme, Microbial Cell Factories, 2018, 17(1):23.

4.      Peng Wen, Dan Wu, Chengpeng Chen, Pu Zhen*. Highly efficient biosynthesis of heliotropin by Escherichia coli engineered to co-express of trans-anethole oxygenase and formate dehydrogenase. Journal of Agricultural and Food Chemistry. 2019, 67, 14121-14128.

5.      Deqing Wang, Pu Zheng*, Pengcheng Chen, and Dan Wu. Highly Efficient Enzymatic Conversion of Rutin to Isoquercitrin and L?Rhamnose Using Deep Eutectic Solvents. 2020, ACS Sustainable Chemistry Engineering. 2020, 8, 14905-14913

6.      Lingqia Su, Yaqi Zhao, Dan Wu, Jing Wu. Heterogeneous expression, molecular modification of amylosucrase from Neisseria polysaccharea, and its application in the preparation of turanose. Food Chemistry, 2020, 314: 126212.

7.     Yang Xu, JingWu, Kaixuan Zheng, Dan Wu*, A xylanase from Streptomyces sp. FA1: heterologous expression, characterization, and its application in Chinese steamed bread, Journal of Industrial Microbiology & Biotechnology, 2016, 43(5): 663-670.

8.      Dan Wu, Ju Chu, YuYou Hao, YongHong Wang, YingPing Zhuang, Si-Liang Zhang. Incomplete protein disulphide bond conformation and decreased protein expression result from high cell growth during heterologous protein expression in Pichia pastoris. Journal of Biotechnology, 2012, 157 (1): 107-112.

9.      Dan Wu, Ju Chu, YuYou Hao, YongHong Wang, YingPing Zhuang, SiLiang Zhang. High efficient production of recombinant human consensus interferon mutant in high cell density culture of Pichia pastoris using two phases methanol control. Process Biochemistry, 2011, 46: 1663-1669.

10.  Dan Wu, Sheng Chen, Ning Wang, Jian Chen, Jing Wu. Gamma-cyclodextrin production using cyclodextrin glycosyltransferase from Bacillus clarkii 7364. Applied Biochemistry and Biotechnology, 2012, 167:1954-1962.

11.  Dan Wu, XiaoWei Yu, TongChun Wang, Rui Wang, and Yan Xu. High Yield Rhizopus chinenisis prolipase Production in Pichia Pastoris: Impact of Methanol Concentration. Biotechnology and Bioprocess Engineering, 2011, 16: 305-311.

12.  Dan Wu, Ma Dong, Hao YuYou, Chu Ju, Wang YongHong, Zhuang YingPing, Zhang SiLiang. Incomplete formation of intramolecular disulfide bond triggers degradation and aggregation of human consensus interferon-α mutant by Pichia pastoris. Applied Microbiology Biotechnology, 2010, 85(6): 1759-67.

13.  Dan Wu, YuYou Hao, Ju Chu, YingPing Zhuang, SiLiang Zhang. Inhibition of degradation and aggregation of recombinant human consensus interferon-α mutant expressed in Pichia pastoris with complex medium in bioreactor. Applied Microbiology Biotechnology, 2008, 80: 1063-1071.

14.  Zhu Li, Lingqia Su, Xuguo Duan, Dan Wu, Jing Wu*, Efficient expression of a maltohexaose-forming α-amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and its use in maltose production, BioMed Research International, 2017, doi:10.1155/2017/5479762

15.  Hongyan Ran, Jing Wu, Dan Wu, Xuguo Duan*, Enhanced Production of Recombinant Thermobifida fusca Isoamylase in Escherichia coli MDS42, Applied Biochemistry and Biotechnology, 2016, 180(3): 464-476

16.  Miao He, Dan Wu, Jing Wu*, Jian Chen, Enhanced expression of endoinulinase from Aspergillus niger by codon optimization in Pichia pastoris and its application in inulooligosaccharide production, Journal of Industrial Microbiology & Biotechnology, 2014, 41(1): 105-114

17.  Xingyi Chen, Lingqia Su, Dan Wu, Jing Wu*, Application of recombinant Bacillus subtilis γ-glutamyltranspeptidase to the production of L-theanine, Process Biochemistry, 2014, 49(9): 1429-1439

18.  Xiaojun sun, Xuguo Duan, Dan Wu, Jing Wu*, Characterization of Sulfolobus solfataricus β-galactosidase mutant F441Y expressed in Pichia pastoris, Journal of the Science of Food and Agriculture, 2014, 94(7): 1359-1365

19.  Hui Deng, Sheng Chen, Dan Wu, Jian Chen, Jing Wu*, Heterologous expression and biochemical characterization of glucose isomerase from Thermobifida fusca, Bioprocess and Biosystems Engineering, 2014, 37(6): 1211-1219.

20.  Fan Zhang, Xuguo Duan, Sheng Chen, Dan Wu, Jian Chen, Jing Wu*, The addition of Co2+ enhances the catalytic efficiency and thermostability of recombinant glucose isomerase from Thermobifida fusca, Process Biochemistry, 2013, 48(10): 1502-1508

21.  Lei Wang, Dan Wu, Jian Chen, Jing Wu*, Enhanced production of γ-cyclodextrin by optimization of reaction of γ-cyclodextrin glycosyltransferase as well as synchronous use of isoamylase, Food Chemistry, 2013, 141(3): 3072-3076

22.  Yufei Wu, Shuguang Yuan, Sheng Chen, Dan Wu, Jian Chen, Jing Wu*, Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus β-galactosidase, Food Chemistry, 2013, 138(2-3): 1588- 1595 

23.  Xu Liu, Dan Wu, Jing Wu*, Jian Chen, Optimization of the production of Aspergillus niger α-glucosidase expressed in Pichia pastoris, World Journal of Microbiology and Biotechnology, 2013, 29(3): 533-540

24.  Berhane T. Tesfai, Dan Wu, Sheng Chen, Jian Chen, Jing Wu*, Effect of organic solvents on the yield and specificity of cyclodextrins by recombinant cyclodextrin glucanotransferase (CGTase) from Anaerobranca gottschalkii, Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2013, 77: 147-153

25.  Berhane T. Tesfai, Dan Wu, Sheng Chen, Jian Chen, Jing Wu*, Strategies for enhancing extracellular secretion of recombinant cyclodextrin glucanotransferase in E. coli, Applied Biochemistry and Biotechnology, 2012, 167(4):897-908

26.  Zhijun Zhao, Sheng Chen, Dan Wu, Jing Wu*, Jian Chen. Effect of gene knockouts of L-tryptophan uptake system on the production of L-tryptophan in Escherichia coli, Process Biochemistry, 2012, 47(2):340-344

27.  Jianhong Zhang, Dan Wu, Jian Chen, Jing Wu*, Enhancing functional expression of beta-glucosidase in Pichia pastoris by co-expressing protein disulfide isomerase,  Biotechnology and Bioprocess Engineering, 2011, 16(6):1196-1200.

28.  Zhijun Zhao, Chun Zou, Yixing Zhu, Jun Dai, Sheng Chen, Dan Wu, Jing Wu*, Jian Chen*, Development of L-tryptophan production strains by defined genetic modification in Escherichia coli, Journal of Industrial Microbiology and Biotechnology, 2011, 38:1921-1929.

29.  Jing Cheng, Dan Wu, Sheng Chen, Jian Chen, Jing Wu*, High-Level Extracellular production of α-cyclodextrin glycosyltransferase with recombinant Escherichia coli BL21(DE3), Journal of Agricultural and Food Chemistry, 2011, 59:3797–3802

30.  Dongli Chen, Xing Tong, Shangwei Chen, Sheng Chen, Dan Wu, Shuguang Fang, Jing Wu*, Jian Chen*. Heterologous expression and biochemical characterization of alpha-glucosidase from Aspergillus niger by Pichia pastroris, Journal of Agricultural and Food Chemistry, 2010, 58(8):4819-4824

31.  Runrong Ding, Zhaofeng Li, Sheng Chen, Dan Wu, Jing Wu*, Jian Chen*, Enhanced secretion of recombinant α-cyclodextrin glucosyltransferase from E. coli by medium additives, Process Biochemistry, 2010, 45:880-886

32.  潘阳, 吴丹, 吴敬*.利用游离型表达质粒强化毕赤酵母表达木聚糖酶. 生物工程学报. 2018, 34(5): 712-721.

33.  陈磊, 陈晟, 吴敬, 吴丹*. 基于体外分子进化技术提高弯曲芽孢杆菌 CCTCC 2015368 β-淀粉酶的热稳定性[J]. 生物工程学报, 2018, 34(2): 255-263.

 

二、专利

1. Jing Wu, Lei Wang, Dan Wu, and Jian Chen. A method for producing γ-cyclodextrin by simultaneous use of γ-cyclodextrin glycosyltransferase and isoamylase. 专利号8,871,473

2. Jian Chen, Jing Wu, Dan Wu, Lei Wang. Cutinase producing genetically engineered microorganism and use thereof. 专利号8,785,169 B2

3. 吴敬,王磊,吴丹,陈坚:一种生物法生产γ-环糊精的生产工艺. 中国, ZL201210347398.7

4. 吴敬,段绪果,吴丹,陈晟,陈坚. 一种耐热酸性普鲁兰酶生产菌株及其筛选方法. 中国, ZL201310079335.2

5. 吴敬,李祝,吴丹,陈晟,陈坚. 一种α-淀粉酶高产菌株及其发酵生产淀粉酶的方法. 中国, ZL201310076922.6

6. 吴敬,王磊,吴丹,陈坚. 一种双酶复配生产γ-环糊精的生产工艺. 中国, ZL201310036791.9

7. 吴敬,刘旭,吴丹,陈坚. 一种黑曲霉α-葡萄糖苷酶基因及其高效表达方法. 2014.06.18,中国,ZL201310095971.4

8. 吴敬,吴丹,程婧,陈坚. 一种胞外生产重组α-环糊精葡萄糖基转移酶的生产工艺. 中国, ZL201010181328.X

9. 吴敬,陈晟,吴丹,赵志军,陈坚. 一种大肠杆菌胞内表达载体及应用, 中国, ZL201010226274.4

10. 吴敬,吴丹,王蕾,陈坚. 一种产角质酶基因工程菌及其应用.中国,ZL201010578924.1

11. 吴敬,吴丹,郑贤良,陈坚,陈晟. 一种环糊精葡萄糖基转移酶复配酶制剂. 2012.05.30,中国,ZL200910260985.0

12. 吴敬,吴丹,王宁,陈坚,陈晟. 一种生物法生产α-环糊精的生产工艺. 2012.10.10,中国,ZL200910260986.5

 

三、承担项目情况

1. “ROS胁迫下的毕赤酵母Mpr1抗氧化分子调控机制”, 江苏省基础研究计划(自然科学基金)-面上项目, 编号: BK20171261, 2017.07-2020.06, 项目负责人

2. “1,3-位置选择性脂肪酶的发酵调控研究”, 国家高技术研究发展计划(863计划)项目, 编号: 2010AA101501, 2010.01- 2011.11, 项目负责人.

3. “优良食品微生物高通量筛选与细胞选育技术”, 国家高技术研究发展计划(863计划)项目, 编号: 2011AA100904, 2011.01- 2013.12, 项目参与人

4. “Pichia pastoris快速合成精氨酸途径中两个关键基因“MPR1/2”PUT1抗氧化胁迫功能”, 华东理工大学生物反应器工程国家重点实验室开放课题, 2011.06.01-2013.05.31, 项目负责人.

5. “毕赤酵母内源性活性氧与外源蛋白高效表达的相关性”, 电玩城赠送金币50万食品科学与技术国家重点实验室自由探索项目, 编号: SKLF-TS-200910, 2009.01.-2010.12, 项目负责人.

6. “重组毕赤酵母高效表达α-, β-葡萄糖苷酶的研究”, 工业微生物教育部重点实验室开放课题, 编号: KLIB-KF200904, 2010.01- 2012.01, 项目负责人.

7.  N-乙酰转移酶和脯氨酸氧化酶在毕赤酵母中快速抗氧化胁迫作用机制, 电玩城赠送金币50万自主科研面上项目, 编号: JUSRP211A05, 2011.09-2013.09, 项目负责人.

8. 食品酶制备及应用的基础研究, 国家杰出青年基金, 编号: 31425020, 项目参与人.

9. “淀粉脱支酶的基因挖掘、功能优化及分泌表达新策略”, 国家自然科学基金, 编号: 31271813, 项目参与人.

 

四、获奖情况

1、环糊精葡萄糖基转移酶的制备与应用”, 教育部科技进步奖一等奖, 2012, 排名第四.

2、2010优秀本科生论文指导教师                获得者:李祝

3、2013年硕士研究生国家奖学金获得者指导教师  获得者:刘旭

4、2015年硕士研究生国家奖学金获得者指导教师  获得者:何淼

5、2018年硕士研究生国家奖学金获得者指导教师  获得者:王淼淼

6、新型高效淀粉脱支酶的创制及工业化应用”, 获中国商业联合会科学技术奖特等奖, 2018, 排名第四.

7、基于游离型载体构建双启动子强化型毕赤酵母真核表达系统”, 第二届全国大学生生命科学竞赛优胜奖, 2018, 证书号CULSC201804278, 指导教师,排名第一.


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